anti-IL-1 beta Antibody
Details
Target of this antibody:
IL-1b
This antibody reacts with:
Mouse
Antibody host:
Rabbit
Form of antibody:
Liquid
Shipping conditions:
gel pack
Concentration of antibody:
0.5 mg/ml
French translation
anticorps
Isotype:
Rabbit IgG
Clonality of antibody:
Polyclonal
Purification method:
Affinity purified
This antibody has been succesfully tested for use in following methods:
Western blot, Neutralization
Immunogene sequence:
E. coli expressed murine IL-1β
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Alternative names of antibody target:
IL-1 beta, IL1 beta, IL 1 beta, Interluekin-1 beta, Interluekin 1 beta, interluekin
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Formulation of this antibody:
30 µg (0.5 mg/ml) affinity purified rabbit anti-murine IL-1β polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5%BSA and 0.01% thimerosal.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Recommended usage:
Western blot analysis (0.5-4 µg/ml). Recombinant murine IL-1β (Cat.# 4129-10) can be used as a positive control. Per researcher’s feedback, the antibody can also be used in neutralization studies. However, the optimal conditions should be determined individually.
Background information:
IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant murine IL-1beta is a 17.5 kDa protein containing 153 amino acid residues.