Human CellExp™ IL-1 RII /CD121b, human recombinant
Details
Tested activity
N/A
Concentration
N/A
Aa sequence
N/A
Recombinant
Yes
Unit
N/A
Binding ability
N/A
NCBI gene number
7850
NCBI gene
IL1R2
Gene source
Human
NCBI number
P27930
Purification
≥95%
Storage condition
-20°C
Shipping under
Gel Pack
Physical appearance
Lyophilized
Source
HEK293 cells
Group
recombinants
Tested applications
SDS-PAGE, N/A
Target
IL-1 RII /CD121b
Other name
CD121b, IL1RB, IL1R2, CDw121b
Synonym
CD121b, IL1RB, IL1R2, CDw121b
Additional source
Recombinants or rec. proteins
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Physical properties
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 38.6 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.
Reesults
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.
Biological activity
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Background
Interleukin-1 receptor type 2 (IL1R2) also known as CD121 antigen-like family member B (CDw121b), IL-1 type II receptor, Interleukin-1 receptor type II, belongs to the interleukin-1 receptor family. Two distinct types of IL1 receptors which are able to bind IL1 specifically have been identified designated as IL1RI (IL1RA) and IL1RII (IL1RB). IL1R2 is non-signaling receptor for IL1A, IL1B and IL1RN, reduces IL1B activities. Serves as a decoy receptor by competitive binding to IL1B and preventing its binding to IL1R1. IL1R2 modulates cellular response through non-signaling association with IL1RAP after binding to IL1B. IL1R2 (membrane and secreted forms) preferentially binds IL1B and poorly IL1A and IL1RN. The secreted IL1R2 recruits secreted IL1RAP with high affinity; this complex formation may be the dominant mechanism for neutralization of IL1B by secreted/soluble receptors.