Human CellExp IL-1 RII /CD121b, human recombinant
Details
Recombinant
Yes
Concentration
N/A
Purity
N/A
Activity (Specifications/test method)
N/A
NCBI Gene ID #.
7850
NCBI Gene Symbol
IL1R2
Gene Source
Human
Accession #
P27930
Shelf Life
1 year
Storage Temp.
-20°C
Shipping
Gel Pack
Appearance
Lyophilized
Group
recombinants
Source
HEK293 cells
Biomolecule/Target
IL-1 RII /CD121b
Synonyms
CD121b, IL1RB, IL1R2, CDw121b
Alternates names
CD121b, IL1RB, IL1R2, CDw121b
Additional source
Recombinants or rec. proteins
Handling
Centrifuge the vial prior to opening.
Usage
For Research Use Only! Not to be used in humans
Taglines
Serves as a decoy receptor by competitive binding to IL1B
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
Molecular Weight
This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 38.6 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.
Results
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.
Biological activity
Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.
Reconstitution Instructions
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.