Human CellExp IL-1 beta / IL-1F2, Human recombinan ...
Details
Recombinant
Yes
Concentration
N/A
Purity
N/A
Biomolecule/Target
IL1B
NCBI Gene ID #.
3553
NCBI Gene Symbol
IL1B
Gene Source
Human
Accession #
P01584
Shelf Life
1 year
Storage Temp.
-20°C
Shipping
Gel pack
Endotoxin Level
< 1 EU/μg
Appearance
Lyophilized
Group
recombinants
Source
HEK 293 cells
Activity (Specifications/test method)
Prolifiration assay
Results
>500,000,000 Unit/mg.
Additional source
Recombinants or rec. proteins
Taglines
Potent proinflammatory cytokine
Handling
Centrifuge the vial prior to opening.
Alternates names
IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin
Synonyms
IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin
Usage
For Research Use Only! Not to be used in humans
Reconstitution Instructions
Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml
Molecular Weight
The protein has a calculated MW of 17.5 kDa. The predicted N-terminus is Met. The reducing protein migrates as 18 kDa in SDS-PAGE.
Physical form description
Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10.G4.1 cells. The ED50 < 2 pg/mL, corresponding to a specific activity of >500,000,000 Unit/mg.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.