IL-1 Receptor Agonist Recombinant Protein
Details
NCBI ID
3557
Protein's origin
Human
Uniprot
P18510
Recombinant in
E. coli
Rec. PROTEIN GI#
10835147
Accession nr
NP_000568.1
Vial
Lyophilized
Group
recombinants
Source
Homo sapiens
Additional source
Recombinants or rec. proteins
Gene name
interleukin 1 receptor antagonist
About
This product is for research use only.
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Other name of the recombinant
DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein
SEQUENCE
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Activity
Biological activity was determined by its ability to inhibit the IL - 1alpha stimulation of murine D10S cell. The expected ED50is 20 - 40 ng/mL in the presence of 50 pg/mL of IL - 1alpha.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Keep at
The lyophilized IL-1 Receptor Agonist recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 Receptor Agonist is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 Receptor Agonist recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant human IL-1Ra is a 17.2 kDa protein consisting of 153 amino acid residues.
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.