Details

Clone Name

AS10

Species Reactivity

Human

Host/Source

Mouse

 

French translation

anticorps

Application

Flow Cytometry

Shipping Conditions

Room Temperature

 

Conjugation

Anti-FITC Antibody

Product Category

Inflammation & Immunology

Purification Method

Protein A/G Chromatography

 

Immunogen

Human recombinant interleukin 1 beta (hIL- 1§)

Storage conditions

Interleukin-1 beta (IL-1 §) FITC, Monoclonal Antibody should be stored at -20ºC. Aliquot to avoid freeze/thaw cycles

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Properties

If you buy Antibodies supplied by Exalpha they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Exalpha Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Exalpha FITC is highly sensitive to pH extremes.