Details

Target of this antibody:

IL-1b

This antibody reacts with:

Mouse

Antibody host:

Rabbit

 

Form of antibody:

Liquid

Shipping conditions:

gel pack

Concentration of antibody:

0.5 mg/ml

 

French translation

anticorps

Isotype:

Rabbit IgG

Clonality of antibody:

Polyclonal

 

Purification method:

Affinity purified

This antibody has been succesfully tested for use in following methods:

Western blot, Neutralization

Immunogene sequence:

E. coli expressed murine IL-1β

 

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

How to handle this antibody:

The antibody solution should be gently mixed before use.

Alternative names of antibody target:

IL-1 beta, IL1 beta, IL 1 beta, Interluekin-1 beta, Interluekin 1 beta, interluekin

 

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Formulation of this antibody:

100 µg (0.5 mg/ml) affinity purified rabbit anti-murine IL-1β polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5%BSA and 0.01% thimerosal.

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Recommended usage:

Western blot analysis (0.5-4 µg/ml). Recombinant murine IL-1β (Cat.# 4129-10) can be used as a positive control. Per researcher’s feedback, the antibody can also be used in neutralization studies. However, the optimal conditions should be determined individually.

Background information:

IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant murine IL-1beta is a 17.5 kDa protein containing 153 amino acid residues.