Human CellExp™ IL-1 Alpha, human recombinant
Details
Concentration
N/A
Recombinant
Yes
Aa sequence
N/A
Reesults
N/A
Binding ability
N/A
Unit
N/A
NCBI gene number
3552
Target
IL-1a
NCBI gene
IL-1a
Gene source
Human
NCBI number
P01583
Purification
≥97%
Storage condition
-20°C
Shipping under
Gel Pack
Group
recombinants
Tested applications
SDS-PAGE, N/A
Source
HEK 293 cells
Physical appearance
Lyophilized powder
Level of endotoxin
< 0.1 ng/μg of protein (
Additional source
Recombinants or rec. proteins
Before use
Centrifuge the vial prior to opening.
Notes
For Research Use Only! Not to be used in humans
Molecular weight
Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE
Description
The CellExp™ IL-1 Alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.
Synonym
IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin
Other name
IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin
Biological activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.
Tested activity
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.
Physical properties
Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.
How to reconstitute
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Background
Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA, IL1F1, and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.