Details

Concentration

N/A

Recombinant

Yes

Aa sequence

N/A

 

Reesults

N/A

Binding ability

N/A

Unit

N/A

 

NCBI gene number

3552

Target

IL-1a

NCBI gene

IL-1a

 

Gene source

Human

NCBI number

P01583

Purification

≥97%

 

Storage condition

-20°C

Shipping under

Gel Pack

Group

recombinants

 

Tested applications

SDS-PAGE, N/A

Source

HEK 293 cells

Physical appearance

Lyophilized powder

 

Level of endotoxin

<0.1 ng/μg of protein (

Additional source

Recombinants or rec. proteins

Before use

Centrifuge the vial prior to opening.

 

Notes

For Research Use Only! Not to be used in humans

Molecular weight

Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE

Description

The CellExp™ IL-1 Alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.

 

Synonym

IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin

Other name

IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.

 

Tested activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.

Physical properties

Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

How to reconstitute

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA, IL1F1, and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.