Details

Concentration

N/A

Aa sequence

N/A

Recombinant

Yes

 

Unit

N/A

Binding ability

N/A

Target

IL1B

 

NCBI gene number

3553

NCBI gene

IL1B

Gene source

Human

 

NCBI number

P01584

Storage condition

-20°C

Purification

≥ 97%

 

Shipping under

Gel pack

Level of endotoxin

<1 EU/μg

Physical appearance

Lyophilized

 

Group

recombinants

Tested applications

SDS-PAGE, N/A

Source

HEK 293 cells

 

Tested activity

Prolifiration assay

Reesults

>500,000,000 Unit/mg.

Additional source

Recombinants or rec. proteins

 

Before use

Centrifuge the vial prior to opening.

Other name

IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin

Synonym

IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin

 

Notes

For Research Use Only! Not to be used in humans

How to reconstitute

Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

Molecular weight

The protein has a calculated MW of 17.5 kDa. The predicted N-terminus is Met. The reducing protein migrates as 18 kDa in SDS-PAGE.

 

Physical properties

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10.G4.1 cells. The ED50 <2 pg/mL, corresponding to a specific activity of>500,000,000 Unit/mg.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

Interleukin-1 beta (IL-1β) is also known as catabolin, is a cytokine protein that in humans is encoded by the IL1B gene. IL-1β precursor is cleaved by caspase 1 (interleukin 1 beta convertase). Cytosolic thiol protease cleaves the product to form mature IL-1 beta. IL1β are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL1α and IL1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.