Details

Purity

N/A

Recombinant

Yes

Results

N/A

 

Concentration

N/A

NCBI Gene ID #.

3552

Biomolecule/Target

IL-1a

 

NCBI Gene Symbol

IL-1a

Gene Source

Human

Accession #

P01583

 

Storage Temp.

-20°C

Shelf Life

1 year

Shipping

Gel Pack

 

Group

recombinants

Source

HEK 293 cells

Appearance

Lyophilized powder

 

Endotoxin Level

<0.1 ng/μg of protein (

Additional source

Recombinants or rec. proteins

Handling

Centrifuge the vial prior to opening.

 

Usage

For Research Use Only! Not to be used in humans

Taglines

Plays one of the central roles in the regulation of the immune responses

Molecular Weight

Calculated MW of 18.0 kDa with no tag. DTT-reduced protein migrates as 22 kDa. in SDS-PAGE

 

Description

The CellExp IL-1 Alpha, recombinant is a α- or alpha protein sometimes glycoprotein present in blood.

Alternates names

IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin

Synonyms

IL1A, IL-1A, IL1, IL1-ALPHA, IL1ALPHA, IL1F1, IL-1α, IL1α, Interleukin-1 alpha, Interleukin-1, Interleukin

 

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.

Activity (Specifications/test method)

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10S cells. The ED50 1X10^9 Unit/mg.

Physical form description

Lyophilized from 0.22 μm filtered solution in in PBS, pH 7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

 

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.