Details

Recombinant

Yes

Concentration

N/A

Purity

N/A

 

Activity (Specifications/test method)

N/A

NCBI Gene ID #.

7850

NCBI Gene Symbol

IL1R2

 

Gene Source

Human

Accession #

P27930

Shelf Life

1 year

 

Storage Temp.

-20°C

Shipping

Gel Pack

Appearance

Lyophilized

 

Group

recombinants

Source

HEK293 cells

Biomolecule/Target

IL-1 RII /CD121b

 

Synonyms

CD121b, IL1RB, IL1R2, CDw121b

Alternates names

CD121b, IL1RB, IL1R2, CDw121b

Additional source

Recombinants or rec. proteins

 

Handling

Centrifuge the vial prior to opening.

Usage

For Research Use Only! Not to be used in humans

Taglines

Serves as a decoy receptor by competitive binding to IL1B

 

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Molecular Weight

This protein is fused with 6×His tag at the C-terminus, has a calculated MW of 38.6 kDa. The predicted N-terminus is Thr 14. DTT-reduced Protein migrates as 50-55 kDa due to glycosylation.

Results

Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.

 

Biological activity

Measured by its ability to inhibit IL-1 beta dependent proliferation in D10.G4.1 mouse helper T cells. Approximately 0.5-3 µg/ml of IL1R2 will inhibit 50% of the biological response due to 50 pg/ml of recombinant human IL-1 beta.

Reconstitution Instructions

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 µg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.