Details

Recombinant

Yes

Concentration

N/A

Purity

N/A

 

Biomolecule/Target

IL1B

NCBI Gene ID #.

3553

NCBI Gene Symbol

IL1B

 

Gene Source

Human

Accession #

P01584

Shelf Life

1 year

 

Storage Temp.

-20°C

Shipping

Gel pack

Endotoxin Level

<1 EU/μg

 

Appearance

Lyophilized

Group

recombinants

Source

HEK 293 cells

 

Activity (Specifications/test method)

Prolifiration assay

Results

>500,000,000 Unit/mg.

Additional source

Recombinants or rec. proteins

 

Taglines

Potent proinflammatory cytokine

Handling

Centrifuge the vial prior to opening.

Alternates names

IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin

 

Synonyms

IL1B, IL-1BETA, IL1F2, IL-1β, Catabolin

Usage

For Research Use Only! Not to be used in humans

Reconstitution Instructions

Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/ml

 

Molecular Weight

The protein has a calculated MW of 17.5 kDa. The predicted N-terminus is Met. The reducing protein migrates as 18 kDa in SDS-PAGE.

Physical form description

Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally Mannitol or Trehalose is added as protectants before lyophilization.

Biological activity

The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse D10.G4.1 cells. The ED50 <2 pg/mL, corresponding to a specific activity of>500,000,000 Unit/mg.

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.