IL-1 alpha Recombinant Protein
Details
NCBI ID
3552
Protein's origin
Human
Uniprot
P01583
Recombinant in
E. coli
Rec. PROTEIN GI#
27894330
Accession nr
NP_000566.3
Vial
Lyophilized
Group
recombinants
Source
Homo sapiens
Gene name
interleukin 1, alpha
Additional source
Recombinants or rec. proteins
About
This product is for research use only.
Other name of the recombinant
IL1, IL-1A, IL1F1, IL1-ALPHA, Interleukin-1 alpha, Hematopoietin-1, IL-1 alpha
Description
The IL-1 alpha Recombinant Protein is a α- or alpha protein sometimes glycoprotein present in blood.
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Activity
The ED50 was determined by the dose - dependent stimulation of murine D10S cells is ≤ 0.001 ng/mL, corresponding to a specific activity of ≥ 1 x 109 units/mg.
SEQUENCE
SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Keep at
The lyophilized IL-1 alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein
IL-1β is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant human IL-1α is an 18.0 kDa protein containing 159 amino acid residues.