Details

NCBI ID

3552

Protein's origin

Human

Uniprot

P01583

 

Recombinant in

E. coli

Rec. PROTEIN GI#

27894330

Accession nr

NP_000566.3

 

Vial

Lyophilized

Group

recombinants

Source

Homo sapiens

 

Gene name

interleukin 1, alpha

Additional source

Recombinants or rec. proteins

About

This product is for research use only.

 

Other name of the recombinant

IL1, IL-1A, IL1F1, IL1-ALPHA, Interleukin-1 alpha, Hematopoietin-1, IL-1 alpha

Description

The IL-1 alpha Recombinant Protein is a α- or alpha protein sometimes glycoprotein present in blood.

Pureity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

 

Activity

The ED50 was determined by the dose - dependent stimulation of murine D10S cells is ≤ 0.001 ng/mL, corresponding to a specific activity of ≥ 1 x 109 units/mg.

SEQUENCE

SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRISKTQLY VTAQDEDQPV LLKEMPEIPK TITGSETNLL FFWETHGTKN YFTSVAHPNL FIATKQDYWV CLAGGPPSIT DFQILENQA

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

Keep at

The lyophilized IL-1 alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

About recombinant protein

IL-1β is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant human IL-1α is an 18.0 kDa protein containing 159 amino acid residues.