Details

NCBI ID

16175

Protein's origin

Murine

Uniprot

P01582

 

Recombinant in

E. coli

Rec. PROTEIN GI#

47059075

Accession nr

NP_034684.2

 

Vial

Lyophilized

Group

recombinants

Source

Mus musculus

 

Gene name

interleukin 1 alpha

Additional source

Recombinants or rec. proteins

Other name of the recombinant

Il-1a, Interleukin-1 alpha, IL-1 alpha

 

About

This product is for research use only.

Description

The IL-1 alpha Recombinant Protein is a α- or alpha protein sometimes glycoprotein present in blood.

Pureity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

 

Activity

The ED50 was determined by the dose - dependent stimulation of murine D10S cells is <0.002 ng/mL, corresponding to a specific activity of> 5 x 108 units/mg.

SEQUENCE

SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

Keep at

The lyophilized IL-1 alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

About recombinant protein

IL-1α is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant murine IL-1α is an 17.9 kDa protein containing 156 amino acid residues.