IL-1 alpha Recombinant Protein
Details
Protein's origin
Rat
NCBI ID
24493
Uniprot
P16598
Recombinant in
E. coli
Rec. PROTEIN GI#
8393593
Accession nr
NP_058715.1
Vial
Lyophilized
Group
recombinants
Source
Rattus norvegicus
Gene name
interleukin 1 alpha
Additional source
Recombinants or rec. proteins
About
This product is for research use only.
Other name of the recombinant
IL-1 alpha, Interleukin-1 alpha, IL-1 alpha
Description
The IL-1 alpha Recombinant Protein is a α- or alpha protein sometimes glycoprotein present in blood.
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
Activity
The ED50 was determined by the dose - dependent proliferation of murine D10S cells is < 0.005 ng/mL, corresponding to a specific activity of > 2 x 108 units/mg.
SEQUENCE
MAPHSFQNNL RYKLIRIVKQ EFIMNDSLNQ NIYVDMDRIH LKAASLNDLQ LEVKFDMYAY SSGGDSKYPV TLKVSNTQLF VSAQGEDKPV LLKEIPETPK LITGSETDLI FFWEKINSKN YFTSAAFPEL LIATKEQSQV HLARGLPSMI DFQIS
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Keep at
The lyophilized IL-1 alpha recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 alpha is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 alpha recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein
IL-1β is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant rat IL-1α is a 17.7 kDa protein containing 155 amino acid residues.