Details

Protein's origin

Rat

NCBI ID

24494

Uniprot

Q63264

 

Recombinant in

E. coli

Rec. PROTEIN GI#

158186736

Vial

Lyophilized

 

Accession nr

NP_113700.2

Group

recombinants

Source

Rattus norvegicus

 

Gene name

interleukin 1 beta

Other name of the recombinant

Interleukin-1 beta, IL-1 beta

Additional source

Recombinants or rec. proteins

 

About

This product is for research use only.

Pureity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Activity

The ED50 was determined by the dose - dependent stimulation of murine D10S cells is <0.1 ng/mL, corresponding to a specific activity of> 1 x 107 units/mg.

 

SEQUENCE

MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Keep at

The lyophilized IL-1 beta recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

 

About recombinant protein

IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. Recombinant rat IL-1beta is a 17.3 kDa protein containing 153 amino acid residues.