IL-1 beta Recombinant Protein
Details
NCBI ID
3553
Protein's origin
Human
Uniprot
P01584
Recombinant in
E. coli
Rec. PROTEIN GI#
10835145
Vial
Lyophilized
Accession nr
NP_000567.1
Group
recombinants
Source
Homo sapiens
Gene name
interleukin 1, beta
Additional source
Recombinants or rec. proteins
About
This product is for research use only.
Other name of the recombinant
IL-1, IL1F2, IL1-BETA, Interleukin-1 beta, Catabolin, IL-1 beta
Pureity
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
SEQUENCE
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
Activity
The ED50 was determined by the dose - dependent stimulation of thymidine uptake by murine D10S cells is ≤ 1.0 pg/mL, corresponding to a specific activity of ≥ 1 x 109 units/mg.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Keep at
The lyophilized IL-1 beta recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 beta is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 beta recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
About recombinant protein
IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine. Recombinant human IL-1β is a 17.3 kDa protein containing 153 amino acid residues.