Recombinants or rec. proteins
interleukin 1 receptor antagonist
This product is for research use only.
Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).
DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein
MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Biological activity was determined by its ability to inhibit the IL - 1alpha stimulation of murine D10S cell. The expected ED50is 20 - 40 ng/mL in the presence of 50 pg/mL of IL - 1alpha.
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
The lyophilized IL-1 Receptor Agonist recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 Receptor Agonist is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 Receptor Agonist recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant human IL-1Ra is a 17.2 kDa protein consisting of 153 amino acid residues.
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.