Details

NCBI ID

3557

Protein's origin

Human

Uniprot

P18510

 

Recombinant in

E. coli

Rec. PROTEIN GI#

10835147

Accession nr

NP_000568.1

 

Vial

Lyophilized

Group

recombinants

Source

Homo sapiens

 

Additional source

Recombinants or rec. proteins

Gene name

interleukin 1 receptor antagonist

About

This product is for research use only.

 

Pureity

Greater than 98% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).

Other name of the recombinant

DIRA, IRAP, IL1F3, IL1RA, MVCD4, IL-1RN, IL-1ra, IL-1ra3, ICIL-1RA, Interleukin-1 receptor antagonist protein

SEQUENCE

MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE

 

Activity

Biological activity was determined by its ability to inhibit the IL - 1alpha stimulation of murine D10S cell. The expected ED50is 20 - 40 ng/mL in the presence of 50 pg/mL of IL - 1alpha.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Keep at

The lyophilized IL-1 Receptor Agonist recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted IL-1 Receptor Agonist is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing IL-1 Receptor Agonist recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.

 

About recombinant protein

Interleukin-1 receptor antagonist (IL-1RA) is a naturally occurring inflammatory inhibitor protein. It inhibits the activity of IL-1α and IL-1β by competitively blocking binding to their type I and type II receptors. IL-1Ra is produced by corneal epithelial cells, monocytes, neutrophils, macrophages, and fibroblasts. Therapeutically, IL-1Ra may help in the treatment of sepsis, cachexia, rheumatoid arthritis, chronic myelogenous leukemia, asthma, psoriasis, and inflammatory bowel disease. Recombinant human IL-1Ra is a 17.2 kDa protein consisting of 153 amino acid residues.

Description

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.