MOUSE Anti-MOUSE IL-1 ALPHA:Azide Free Antibody
Details
Gene name synonims
N/A
Purification method
N/A
Immunoglobulin isotype
IgG1
Clone
Flo1
French translation
anticorps
Category
Antibodies
Gene name
IL-1 ALPHA
Clonality
Monoclonal
Also known as
IL-1 ALPHA
Latin name
Mus musculus
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Concentration
IgG concentration 1.0mg/ml
Other gene names
Il1a; Il1a; Il-1a; IL-1 alpha
Form/Appearance
Azide Free (Purified IgG - liquid)
Tested applications:
ELISA (EIA), Functional Assay (FA)
Other names
interleukin-1 alpha; Interleukin-1 alpha; interleukin-1 alpha; IL-1 alpha; interleukin 1 alpha; N/A
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Storage and shipping
Store the antibody should be stored at -20 degrees Celsius. only. Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Description
The MOUSE Anti-MOUSE IL-1 ALPHA: Antibody is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request.Recently MBS Monoclonals made this MOUSE Anti-MOUSE IL-1 ALPHA: Antibody available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.
Specificity and cross-reactivity
IL-1 ALPHA This item specifically recognises mouse interleukin-1 alpha (IL-1 alpha), a pleiotropic pro-inflammatory cytokine and member of the IL-1 superfamily, secreted by activated macrophages and monocytes, which mediates a wide range of immune and inflammatory responses. _x000D__x000D_IL-1 alpha and closely related IL-1 beta, are both involved in haematopoiesis and exhibit many identical immunomodulatory properties, binding to the same type I interleukin-1 receptor, also known as CD121a. _x000D__x000D_Clone Flo1 can be used in functional studies to neutralize the activity of 125pg/ml of recombinant mouse IL-1 alpha by 95%, as tested using mouse D10(N4)M T-helper cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.