Details

Gene ID number

3553

Conjugation

FITC

Modification site

None

 

Swiss Prot

P01584

Subcellular location

Secreted

Target Antigen

IL-1 Beta

 

Tested applications

IF(IHC-P)

French translation

anticorps

Modification

Unmodified

 

Clonality

Polyclonal

Concentration

1ug per 1ul

Excitation Emission

494nm/518nm

 

Immunogen_range

190-240/269

Crossreactivity

Human, Mouse, Rat

Additional conjugation

Anti-FITC Antibody

 

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Category

Conjugated Primary Antibodies

 

Host Organism

Rabbit (Oryctolagus cuniculus)

Purification method

This antibody was purified via Protein A.

Long name

IL-1 Beta Polyclonal Antibody, FITC Conjugated

 

Specificity

This is a highly specific antibody against IL-1 Beta.

Source

KLH conjugated synthetic peptide derived from human IL-1 Beta

Synonyms

IL-1; IL1F2; IL1-BETA; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B

 

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

 

Antigen background

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.