Human IL-1 alpha PicoKine ELISA Kit
Details
Entrez nr.
3552
Minutes of TMB incubation
20-25
Gen protein identity
P01583
ELISA's sensitivity
< 0.5pg/ml
Antigen
E.coli,S113-A271
Gene tested
Human IL-1 alpha
Uniprot
Interleukin-1 alpha
ELISA kit detection limits
4.7pg/ml to 300pg/ml
Gene name
interleukin 1, alpha
Anti-coagulation
heparin, EDTA or citrate
Target of this ELISA
Natural and recombinant human IL-1 alpha
Test
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Detector and capure antibodies
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Cross reaction
the ELISA does not cross react with any other proteins except from closely related species
Description
The IL-1 alpha PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.
Keep at
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
ELISA's application
For quantitative measurement of human IL-1 alpha in cell culture supernatant, serum and plasma heparin, EDTA, citrate .
ELISA standard curve
Concentration(pg/ml) + 0 + 4.7 + 9.4 + 18.8 + 37.5 + 75 + 150 + 300 O.D. + 0.035 + 0.084 + 0.137 + 0.227 + 0.403 + 0.753 + 1.257 + 2.122
ELISA's precision parameters
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);54;147;221;48;139;234 ELISA standard deviations 1.84;7.497;9.28;2.64;10.29;14.98 CV(%);3.4;5.1;4.2;5.5;7.4;6.4
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Alternative
Hematopoietin 1;IL 1 alpha;Il 1a;IL1;IL1 ALPHA;IL1A;IL1F1;Ilia;Interleukin 1 alpha;Interleukin 1 alpha precursor;Interleukin1 alpha;OTTHUMP00000203692;OTTMUSP00000016430;Preinterleukin 1;Pro interleukin 1;Pro interleukin 1 alpha;Prointerleukin 1;RP23-160G19.8
The ELISA contains
Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-1 alpha antibodies;1 ST0000-10;Lyophilized recombinant human IL-1 alpha standard;10ng/tubex2 AR1107;Biotinylated anti- human IL-1 alpha antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Properties
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA kit description
GENTAUR's human IL-1 alpha ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S113-A271) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 alpha will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-1 alpha in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
How the elisa works
Sample the amount of 100ul/well of the standard solution at 300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.8pg/ml, 9.4pg/ml, 4.7pg/ml human IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human IL-1 alpha standard solution and each sample is measured in duplicate.
More gene info
Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant human IL-1alpha with the molecular mass of 18kDa.