Details

Anti-coagulation

/

Entrez nr.

24493

Minutes of TMB incubation

25-30

 

Gen protein identity

P16598

ELISA's sensitivity

<0.5pg/ml

Gene tested

Rat IL-1 alpha

 

Antigen

E.coli,S115-S270

Latin name

Rattus norvegicus

Uniprot

Interleukin-1 alpha

 

Gene name

interleukin 1, alpha

ELISA kit detection limits

4.69pg/ml to 300pg/ml

Target of this ELISA

Natural and recombinant rat IL-1 alpha

 

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

ELISA's application

For quantitative measurement of rat IL-1 alpha in cell culture supernatant and serum.

 

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Description

The IL-1 alpha PicoKine ELISA Kit is a α- or alpha protein sometimes glycoprotein present in blood.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

 

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 4.69 + 9.4 + 18.8 + 37.5 + 75 + 150 + 300 O.D. + 0.040 + 0.110 + 0.157 + 0.306 + 0.588 + 1.013 + 1.552 + 2.148

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);45;137;211;49;145;230 ELISA standard deviations 1.89;6.58;10.76;2.646;8.99;15.41 CV(%);4.2;4.8;5.1;5.4;6.2;6.7

 

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- rat IL-1 alpha antibodies;1 ST0000-10;Lyophilized recombinant rat IL-1 alpha standard;10ng/tubex2 AR1107;Biotinylated anti- rat IL-1 alpha antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

 

How the elisa works

Sample the amount of 100ul/well of the standard solution at 300pg/ml, 150pg/ml, 75pg/ml, 37.5pg/ml, 18.8pg/ml, 9.4pg/ml, 4.69pg/ml rat IL-1 alpha standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatant or serum to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each rat IL-1 alpha standard solution and each sample is measured in duplicate.

ELISA kit description

GENTAUR's rat IL-1 alpha ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-1 alpha has been precoated onto 96-well plates. Standards (E.coli,S115-S270) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 alpha will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat IL-1 alpha in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

Alternative

Hematopoietin 1;_x000D_IFL;_x000D_IFN;_x000D__x000D__x000D_IFN alpha;_x000D_IFN-alpha-1/13;_x000D_IFNA@;_x000D_IFNA1;_x000D_IFNA1_HUMAN;_x000D_IFNA13;_x000D_IFNAP22;_x000D_IL 1 alpha;_x000D_Il 1a;_x000D_IL1;_x000D_IL1 ALPHA;_x000D_IL1A;_x000D_IL1F1;_x000D_Ilia;_x000D_Interferon Alpha;_x000D_Interferon alpha 1;_x000D_Interferon alpha 1/13;_x000D_Interferon alpha 1/13 precursor;_x000D_Interferon alpha 13;_x000D_Interferon alpha D;_x000D_Interferon alpha leukocyte;_x000D_Interferon alpha-1/13;_x000D_Interferon alpha-D;_x000D_Interferon leukocyte;_x000D_Interleukin 1 alpha;_x000D_Interleukin 1 alpha precursor;_x000D_Interleukin1 alpha;_x000D_LeIF D;_x000D_MGC138207;_x000D_MGC138505;_x000D_MGC138507;_x000D_OTTHUMP00000203692;_x000D_OTTMUSP00000016430;_x000D_Preinterleukin 1;_x000D_Pro interleukin 1;_x000D_Pro interleukin 1 alpha;_x000D_Prointerleukin 1;_x000D_RP23-160G19.8

 

More gene info

Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL)-1alpha and IL-1beta have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant rat IL-1alpha with the molecular mass of 18kDa.