Details

Entrez nr.

3553

Minutes of TMB incubation

15-20

Gen protein identity

P01584

 

ELISA's sensitivity

<0.15pg/ml

Gene tested

Human IL-1 beta

Antigen

E.coli,A117-S269

 

Uniprot

Interleukin-1 beta

Gene name

interleukin 1, beta

ELISA kit detection limits

3.9pg/ml to 250pg/ml

 

Anti-coagulation

heparin, EDTA or citrate

Target of this ELISA

Natural and recombinant human IL-1 beta

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

 

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

 

ELISA's application

For quantitative measurement of human IL-1 beta in cell culture supernatant, serum and plasma heparin, EDTA, citrate .

ELISA standard curve

Concentration(pg/ml) + 0 + 3.9 + 7.8 + 15.6 + 31.2 + 62.5 + 125 + 250 O.D. + 0.003 + 0.086 + 0.161 + 0.292 + 0.557 + 1.029 + 1.617 + 2.222

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);19;41.2;116;27.5;61.3;179.1 ELISA standard deviations 1.0;2.6;6.5;1.6;4.1;12.7 CV(%);5.3;6.3;5.6;5.8;6.7;7.1

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Alternative

Catabolin;_x000D_Hematopoietin 1;_x000D__x000D__x000D_IFN beta inducing factor;_x000D_IL 1;_x000D_IL 1 beta;_x000D_IL 1B;_x000D_IL-1 beta;_x000D_IL1;_x000D_IL1 BETA;_x000D_IL1B;_x000D_IL1B_HUMAN;_x000D_IL1F2;_x000D_Interleukin 1 beta;_x000D_Interleukin 1 beta precursor;_x000D_Interleukin-1 beta;_x000D_LAF;_x000D_OAF;_x000D_Osteoclast activating factor;_x000D_Preinterleukin beta;_x000D_Pro interleukin 1 beta

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- human IL-1 beta antibodies;1 ST0000-1;Lyophilized recombinant human IL-1 beta standard;1ng/tubex2 AR1107;Biotinylated anti- human IL-1 beta antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

 

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

ELISA kit description

GENTAUR's human IL-1 beta ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,A117-S269) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 beta will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human IL-1 beta in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 16.5pg/ml, 7.8pg/ml, 3.9pg/ml human IL-1 betastandard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatants, serum or plasma(heparin, EDTA, citrate) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each human IL-1 betastandard solution and each sample is measured in duplicate.

 

More gene info

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.