Details

Minutes of TMB incubation

25-30

Entrez nr.

24494

Gen protein identity

Q63264

 

ELISA's sensitivity

<1pg/ml

Gene tested

Rat IL-1 beta

Anti-coagulation

heparin or EDTA

 

Antigen

E.coli,V117-S268

Latin name

Rattus norvegicus

Uniprot

Interleukin-1 beta

 

Gene name

interleukin 1, beta

ELISA kit detection limits

31.2pg/ml to 2000pg/ml

Target of this ELISA

Natural and recombinant rat IL-1 beta

 

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Detector and capure antibodies

a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

 

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 31.2 + 62.5 + 125 + 250 + 500 + 1000 + 2000 O.D. + 0.022 + 0.072 + 0.136 + 0.213 + 0.364 + 0.779 + 1.626 + 2.440

 

ELISA's application

For quantitative measurement of rat IL-1 beta in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);105;756;1359;112;688;1452 ELISA standard deviations 3.99;34.78;66.59;5.712;37.84;92.93 CV(%);3.8;4.6;4.9;5.1;5.5;6.4

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Alternative

Catabolin;_x000D_Hematopoietin 1;_x000D__x000D__x000D_IFN beta inducing factor;_x000D_IL 1;_x000D_IL 1 beta;_x000D_IL 1B;_x000D_IL-1 beta;_x000D_IL1;_x000D_IL1 BETA;_x000D_IL1B;_x000D_IL1B_HUMAN;_x000D_IL1F2;_x000D_Interleukin 1 beta;_x000D_Interleukin 1 beta precursor;_x000D_Interleukin-1 beta;_x000D_LAF;_x000D_OAF;_x000D_Osteoclast activating factor;_x000D_Preinterleukin beta;_x000D_Pro interleukin 1 beta

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- rat IL-1 beta antibodies;1 ST0000-10;Lyophilized recombinant rat IL-1 beta standard;10ng/tubex2 AR1107;Biotinylated anti- rat IL-1 beta antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

 

ELISA kit description

GENTAUR's rat IL-1 beta ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V117-S268) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 beta will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more rat IL-1 beta in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

How the elisa works

Sample the amount of 100ul/well of the standard solution at 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml rat IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each rat IL-1 betastandard solution and each sample is measured in duplicate.

More gene info

The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).