Details

Minutes of TMB incubation

25-30

Entrez nr.

16176

Gen protein identity

P10749

 

ELISA's sensitivity

<1pg/ml

Latin name

Mus musculus

Anti-coagulation

heparin or EDTA

 

Antigen

E.coli,V118-S269

Gene tested

murine IL-1 beta

Uniprot

Interleukin-1 beta

 

Gene name

interleukin 1, beta

ELISA kit detection limits

12.5pg/ml to 800pg/ml

Target of this ELISA

Natural and recombinant mouse IL-1 beta

 

Cross reaction

the ELISA does not cross react with any other proteins except from closely related species

Detector and capure antibodies

A rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.

Keep at

Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

 

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

ELISA standard curve

Concentration(pg/ml) + 0 + 12.5 + 25 + 50 + 100 + 200 + 400 + 800 O.D. + 0.063 + 0.107 + 0.143 + 0.214 + 0.390 + 0.702 + 1.285 + 2.092

ELISA's application

For quantitative measurement of mouse IL-1 beta in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .

 

ELISA's precision parameters

intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);86;376;587;79;346;558 ELISA standard deviations 6.97;19.55;24.07;7.03;25.6;37.94 CV(%);8.1;5.2;4.1;8.9;7.4;6.8

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Alternative

Catabolin;_x000D_Hematopoietin 1;_x000D__x000D__x000D_IFN beta inducing factor;_x000D_IL 1;_x000D_IL 1 beta;_x000D_IL 1B;_x000D_IL-1 beta;_x000D_IL1;_x000D_IL1 BETA;_x000D_IL1B;_x000D_IL1B_HUMAN;_x000D_IL1F2;_x000D_Interleukin 1 beta;_x000D_Interleukin 1 beta precursor;_x000D_Interleukin-1 beta;_x000D_LAF;_x000D_OAF;_x000D_Osteoclast activating factor;_x000D_Preinterleukin beta;_x000D_Pro interleukin 1 beta

 

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

The ELISA contains

Catalog number;Description;Quantity -;96-well plate precoated with anti- mouse IL-1 beta antibodies;1 ST0000-10;Lyophilized recombinant mouse IL-1 beta standard;10ng/tubex2 AR1107;Biotinylated anti- mouse IL-1 beta antibodies;130ul(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex(ABC);130ul(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4

ELISA kit description

GENTAUR's mouse IL-1 beta ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for IL-1 beta has been precoated onto 96-well plates. Standards (E.coli,V118-S269) and your samples are measured with a biotin conjugated polyclonal from goat specific for IL-1 beta will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse IL-1 beta in your sample the higher the ELISA signal will be compared to this elisa's standard curve.

 

How the elisa works

Sample the amount of 100ul/well of the standard solution at 800pg/ml, 400pg/ml, 200pg/ml, 100pg/ml, 50pg/ml, 25pg/ml, 12.5pg/ml mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. See “Sample dilutions Guideline” above for details. We recommend that each mouse IL-1 betastandard solution and each sample is measured in duplicate.

More gene info

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.