Details

Clone

AS10

Antibody is raised in

Mouse

Antibody's reacts with

Human

 

Storage

-20ºC

Protein number

see ncbi

Latin name

Mus musculus

 

Antibody's specificity

No Data Available

Category

Primary Antibodies

Relevant references

no information yet

 

Antibody come from

Full length recombinant human IL 1 beta protein

Antibody's reacts with these species

This antibody doesn't cross react with other species

Antigen-antibody binding interaction

Mouse anti Interleukin-1 beta (IL-1 b) (Neutralizing) Antibody

 

Application

Immunoprecipitation, Immunohistochemistry, Neutralization, Enzyme Immunoassay

Other description

Purified IgG, sterile filtered in phosphate buffered saline, pH 7.4. Protein A/G Chromatography

Long description

Recognizes human IL-1 beta. Does not recognize mouse IL-1 beta, human IL-1 alpha or a panel of other human cytokines.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Warnings

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.

 

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.To cause (an acid or base) to undergo neutralization.

Antibody's suited for

Optimal concentration should be evaluated by serial dilutions._x000B_Neutralization: This antibody has been used at a 100-fold molar excess to neutralize ³95% of the biological activity of human IL-1 . _x000B_Immunohistochemistry: This antibody has been used at 1-10 µg/ml to stain human hIL- 1 expressed in mononuclear cells, following fixation with 4% paraform in PBS for 10 minutes at 4¡C, plus 0.1% saponin 20 minutes RT. _x000B_Immunoprecipitation: This antibody has been used to immunoprecipitate>90% hIL-1 in solution using a double-antibody method. (Midgley, AR and Hepburn, MR, Methods Enzymol., 70, 266, 1980). _x000B_ELISA: This antibody has been used as a solution phase antibody at 2 µg/ml _x000B_in a two site ELISA with biotin conjugated IL-1beta antibody (Cat. No. _x000B_L142M). Typically the ELISA has a working range of 0-500 pg/ml and a _x000B_sensitivity of 5 pg/ml with the appropriate standard. Recommended sample _x000B_volumes are 25-200 µl. Recovery of hIL-1( in human serum is typically>85% in the ELISA _x000B__x000B_ _x000B_