IL-1 Antibody
Details
Recognised antigen
IL-1
Uniprot #
P01583
Category
Antibody
French translation
anticorps
Conjugation
Unconjugated
Purity
Antigen affinity
Clone
Polyclonal antibody
Tested applications
WB, IHC, FACS, ELISA
Form
Antigen affinity purified
Clonality
Polyclonal (rabbit origin)
Host animal
Rabbit (Oryctolagus cuniculus)
Concentration
In 1X PBS, pH 7.4, with 0.09% sodium azide
Recommended dilutions
Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
Intented use
This IL-1 antibodyis to be used only for research purposes and not for diagnostics..
Immunogen
A portion of amino acids 177-206 from human IL1A was used as the immunogen for this IL-1 antibody.
Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Species reactivity
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Notes
The stated application concentrations are suggested starting amounts. Titration of the IL-1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Storage
Store at 4oC for up to one month. For long term, aliquot the IL-1 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
Description
IL1A is produced by activated macrophages and stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]