Details

Gene ID

3553

Modification site

None

Swiss Prot

P01584

 

Subcellular locations

Secreted

Target Protein/Peptide

IL-1 Beta

Applications

IF(IHC-P)

 

Excitation emission

590nm/617nm

Concentration

1ug per 1ul

Conjugated with

ALEXA FLUOR® 594

 

Conjugated

Alexa conjugate 1

Clonality

Polyclonal Antibody

Applications with corresponding dilutions

IF(IHC-P)(1:50-200)

 

Clone

Polyclonal Antibodies

Purification method

Purified by Protein A.

Other name

Anti-IL-1 Beta Polyclonal

 

Group

Polyclonals and antibodies

Type

Conjugated Primary Antibody

Also known as

IL-1 Beta Polyclonal Antibody

 

Conjugation

Alexa Fluor,ALEXA FLUOR® 594

Host organism

Rabbit (Oryctolagus cuniculus)

Properties

For facs or microscopy Alexa 1 conjugate.

 

Specificity

This antibody reacts specifically with IL-1 Beta

Modification

No modification has been applied to this antibody

Antigen Source

KLH conjugated synthetic peptide derived from human IL-1 Beta

 

Cross reactive species

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)

Synonyms

IL-1; IL1F2; IL1-BETA; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B

Storage

Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.

 

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Cross Reactive Species details

No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.

 

Gene

The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.

Background information

Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.

Advisory

Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.