Anti-IL-1 Beta (Polyclonal), ALEXA Fluor 594
Details
Gene ID
3553
Modification site
None
Swiss Prot
P01584
Subcellular locations
Secreted
Target Protein/Peptide
IL-1 Beta
Applications
IF(IHC-P)
Excitation emission
590nm/617nm
Concentration
1ug per 1ul
Conjugated with
ALEXA FLUOR® 594
Conjugated
Alexa conjugate 1
Clonality
Polyclonal Antibody
Applications with corresponding dilutions
IF(IHC-P)(1:50-200)
Clone
Polyclonal Antibodies
Purification method
Purified by Protein A.
Other name
Anti-IL-1 Beta Polyclonal
Group
Polyclonals and antibodies
Type
Conjugated Primary Antibody
Also known as
IL-1 Beta Polyclonal Antibody
Conjugation
Alexa Fluor,ALEXA FLUOR® 594
Host organism
Rabbit (Oryctolagus cuniculus)
Properties
For facs or microscopy Alexa 1 conjugate.
Specificity
This antibody reacts specifically with IL-1 Beta
Modification
No modification has been applied to this antibody
Antigen Source
KLH conjugated synthetic peptide derived from human IL-1 Beta
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Synonyms
IL-1; IL1F2; IL1-BETA; Interleukin-1 beta; IL-1 beta; Catabolin; IL1B
Storage
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Gene
The Interleukin-1 family (IL-1 family) is a group of 11 cytokines, which plays a central role in the regulation of immune and inflammatory responses to infections or sterile insults. Rec. E. coli interleukin-1 for cell culture or antibody production.
Background information
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Advisory
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.